Affinity chromatography media
Affinity chromatography media:
Affinity chromatography is a method of chromatography to separate substances based on specific interactions between biomolecules, such as the binding of enzymes to substrates, receptors to ligands, antibodies to antigens, etc. Such bindings are both specific and reversible. Protein purification is achieved through this reversible binding and separation.
Features of Affinity Chromatography:
—High efficiency, speediness; High selectivity; High resolution; Over 90% purity can be achieved usually through one-step purification; High recovery rate
Composition of Affinity Medium:
—Base Frame:Inert materials coupled with ligands, such as Bestarose FF, Bestarose HP, etc.
—Connecting Arm:Reduce the steric-hindrance effect, greatly increase the adsorption efficiency of ligands and biomacromolecules to be separated;
—Ligands:Substances that interact specifically with a target molecule.
Product Series | Product |
Alkali-resistant antibody affinity medium | AT Protein A Diamond / AT Protein Diamond Plus |
Antibody affinity media | Protein A Diamond / rProtein A Bestarose 4FF |
Antibody affinity media | Protein G Bestarose 4FF |
Nucleic acid affinity medium | Plasmid Cap Bestarose HP/Plasmid Cap Mustang |
Thiophilic affinity medium | IgM Cap Mustang/IgY Cap Mustang |
IgM Capture Bestarose FF/IgM Capture Bestarose HP | |
IgY Capture Bestarose HP | |
Metal chelating affinity media | Ni Bestarose HP/Ni Bestarose FF |
Benzamidine affinity medium | Benzamidine Bestarose 4FF/Benzamidine Bestarose 6B |
Glutathione Affinity Medium | GST Bestarose 4FF/GST Bestarose 4B |
Blue Gum Affinity Medium | Blue Bestarose FF/Blue Bestarose HP |
Diamond Blue/Diamond Blue Mustang | |
Heparin affinity medium | Heparin Bestarose HP/Heparin Bestarose FF |
Endotoxin affinity medium | Endotoxin Cap Bestarose 4FF |
Metal chelating affinity media | IMAC Bestarose HP/IMAC Bestarose FF |
Chelating Bestarose FF | |
Pre-activated affinity media | CNBr-activiated Bestarose 4B/CNBr-activiated Bestarose 4FF |
NHS-activiated Bestarose 4FF | |
Epoxy-activiated Bestarose 6B |
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